Chlorophyll fluorescence as a new marker for peanut seed quality evaluation


  • Julia Marconato Sudki Centro de Energia Nuclear na Agricultura
  • Anna Clara ´Parisi Absalonsen Kimura da Silva Scientific Initiation Student – Center for Nuclear Energy in Agriculture (CENA/USP). E-mail:
  • Valter Arthur Ph.D. in Entomology – Center for Nuclear Energy in Agriculture (CENA/USP). E-mail:
  • Clíssia Barboza da Silva Ph.D. in Crop Science – Center for Nuclear Energy in Agriculture (CENA/USP). E-mail:



seed vigor, image analysis, non-destructive methods


Recent advances in optical imaging technologies have contributed to rapid, accurate and non-destructive analysis in the context of seed quality. This work aimed to verify the potential of chlorophyll fluorescence images as a new marker for analyzing the physiological potential of peanut seeds. The fluorescence signals of chlorophyll a and b were detected in the excitation/emission combinations of 630/700 nm and 405/600 nm, respectively, using seeds artificially aged for 0, 16, 24 and 48 h. The images were captured using a VideometerLab4 equipment (Videometer A/S, Herlev, Denmark), and its software version 5.4.6. Data were compared with the traditional tests used to evaluate the physiological performance of peanut seeds, such as germination tests, electrical conductivity, seedling emergence and emergence speed index. The results revealed that the fluorescence intensity of chlorophyll a and b was lower in seeds with lower vigor. Therefore, the deterioration process of peanut seeds is accompanied by a breakdown of chlorophyll molecules, and consequently, changes in the fluorescent properties of seeds. From a practical point of view, chlorophyll fluorescence images can be successfully used to discriminate high-and low-vigor seed lots, quickly, accurately and non-destructively.


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Anais do XVIII Encontro Sobre a Cultura do Amendoim - 2021